LOCUS       sequence-86613-e        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ERCC6A-c055
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      486..533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      570..1027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(657..676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggagcc tagcgcccag
      121 gcccttgagc tgcagggttt gggtgtggac gtctatgacc aggacgtgct ggaacaggga
      181 gtgcttcagc aggtggacaa tgccatccat gaggccagcc gtgcctccca gctcgttgac
      241 gtggagaagg agtatcggtc ggtcctggat gacctcacgt catgtacgac atccctaagg
      301 caaatcaata aaattattga acagcttagc cctcaatgac agtaaaggtg gatacggatc
      361 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      541 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      841 gacgttggag tccacgttct ttaatagtgg actcttgttc ccaaactgga acaacactca
      901 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      961 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaaat attaacgctt
     1021 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1081 aaatacattc aaatatgtat ccgctcatga attaa
//
