LOCUS       sequence-86615-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ETV4A-c072
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             465..482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(535..553)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      549..596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(720..739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcgggg tgccctgcag
      121 ctgtggcaat ttctggtggc cttgctggat gacccaacaa atgcccattt cattgcctgg
      181 acgggccggg gaatggagtt caagctcatt gagcctgagg aggtcgccag gctctggggc
      241 atccagaaga accggccagc catgaattac gacaagctga gccgctcgct ccgatactat
      301 tatgagaaag gcatcatgca gaaggtggct ggtgagcgtt acgtgtacaa gtttgtgtgt
      361 gagcccgagg ccctcttctc tttggccttc ccggacaatt gacagtaaag gtggatacgg
      421 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      481 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      541 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      601 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      661 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      721 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      781 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      841 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      901 tttgacgttg gagtccacgt tctttaatag tggactcttg ttcc
//
