LOCUS       sequence-86624-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9 VP64-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(89..100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt cattttcttg
       61 acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga tcttgtcaac
      121 actttattat caatagaatc atctttgaga aaagactggg gcacgatggc atccacgtcg
      181 tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct gccgttctgc
      241 aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg gtgttcctta
      301 aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct ttccctactg
      361 ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac gatattctcg
      421 ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt ctgcagtatt
      481 ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag actgtccccc
      541 tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc atcatggatc
      601 aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag gattgtcttt
      661 ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc ccatcctgta
      721 tatcggcgcc tcttgagctg tttcatga
//
