LOCUS       sequence-86626-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9 VP64-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..69)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(79..228)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(241..261)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttgctcacg gtaccagggc cgggattctc ctccacgtca ccgcatgtta gaagacttcc
       61 tctgccctcg ctagcgatta acatatcgag atcgaaatcg tccagagcat cggagccgag
      121 catgtccaga tcaaagtcat ccaatgcgtc gcttcccaac atatccaggt caaagtcatc
      181 gagggcatca gaacccagca tgtcgagatc gaaatcgtcc agcgcgtcgg cgcggctagc
      241 caccttcctc ttcttcttgg ggtcagccct gctgtctcca ccgagctgag agaggtcgat
      301 tcttgtttca tagagccccg taattgactg atgaatcagt gtggcgtcca ggacctcctt
      361 tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag tacttgaagg ctgcaggcgc
      421 gcccaagttg gtcagagtaa acaagtggat aatgttttct gcctgctccc tgatgggctt
      481 atccctgtgc ttattgtaag cagaaagcac cttatcgagg ttagcgtcgg cgaggatcac
      541 tcttttggag aattcgctta tttgctcgat gatctcatca aggtagtgtt tgtgttgttc
      601 cacgaacagc tgcttctgct cattatcttc gggagaccct ttgagctttt catagtggct
      661 ggccagatac aagaaattaa cgtatttaga gggcagtgcc agctcgttac ctttctgcag
      721 ctcgcccgca ctagcgagca ttcgtttccg gccgttttca agctcaaaga gagagtactt
      781 gggaagctta atgatgaggt cttttttgac ctcttt
//
