LOCUS       sequence-86628-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SOCS2A-c016
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             672..689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(742..760)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      756..803
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcaggc ggcgcgtctg
      121 gcgaaggccc tgcgggagct cggtcagaca ggatggtact ggggaagtat gactgttaat
      181 gaagccaaag agaaattaaa agaggcacca gaaggaactt tcttgattag agatagctcg
      241 cattcagact acctactaac aatatctgtt aaaacatcag ctggaccaac taatcttcga
      301 atcgaatacc aagacggaaa attcagattg gactctatca tatgtgtcaa atccaagctt
      361 aaacaatttg acagtgtggt tcatctgatc gactactatg ttcagatgtg caaggataag
      421 cggacaggtc cagaagcccc ccggaacggc actgttcacc tttatctgac caaaccgctc
      481 tacacgtcag caccatctct gcagcatctc tgtaggctca ccattaacaa atgtaccggt
      541 gccatctggg gactgccttt accaacaaga ctaaaagatt acttggaaga atataaattc
      601 caggtatgac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg
      661 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      721 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      781 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      901 tacacttgcc agcgccctag cgcccgctcc tt
//
