LOCUS       sequence-86630-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against mouse Mek1 from puromycin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pSUPER-retro-puro-tetO-Mek1-shRNA
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     58..74
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     59..75
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(209..228)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        374..873
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(409..432)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     806..825
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(898..917)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat cgatctctcg
       61 aggtcgacgg tatcgataag cttaaaaagc ctctcagctc atatggaatt ctcttgaaat
      121 tccatatgag ctgagaggcg gggatctcta tcactgatag ggaacttata agattcccaa
      181 atccaaagac atttcacgtt tatggtgatt tcccagaaca catagcgaca tgcaaatatt
      241 gcagggcgcc actcccctgt ccctcacagc catcttcctg ccagggcgca cgcgcgctgg
      301 gtgttcccgc ctagtgacac tgggcccgcg attccttgga gcgggttgat gacgtcagcg
      361 ttcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
      421 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
      481 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
      541 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
      601 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
      661 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
      721 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
      781 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
      841 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagcta gcttaccatg
      901 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
      961 accctcgccg ccgcgttcgc cgactacccc gccacggcgg cca
//
