LOCUS       sequence-86631-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt tcattttctt
       61 gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg atcttgtcaa
      121 cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatgg catccacgtc
      181 gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc tgccgttctg
      241 caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg ggtgttcctt
      301 aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc tttccctact
      361 gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa cgatattctc
      421 gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg tctgcagtat
      481 tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa gactgtcccc
      541 ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt catcatggat
      601 caactgcatg aagttccggt tggca
//
