LOCUS       sequence-86635-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DYRK2A-c023
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggggaa ggtgaaagcc
      121 acccccatga cacctgaaca agcaatgaag caatacatgc aaaaactcac agccttcgaa
      181 caccatgaga ttttcagcta ccctgaaata tatttcttgg gtctaaatgc taagaagcgc
      241 cagggcatga caggtgggcc caacaatggt ggctatgatg atgaccaggg atcatatgtg
      301 caggtgcccc acgatcacgt ggcttacagg tatgaggtcc tcaaggtcat tgggaagggg
      361 agctttgggc aggtggtcaa ggcctacgat cacaaagtcc accagcacgt ggccctaaag
      421 atggtgcgga atgagaagcg cttccaccgg caagcagcgg aggagatccg aatcctggaa
      481 cacctgcgga agcaggacaa ggataacaca atgaatgtca tccatatgct ggagaatttc
      541 accttccgca accacatctg catgacgttt gagctgctga gcatgaacct ctatgagctc
      601 atcaagaaga ataaattcca gggcttcagt ctgcctttgg ttcgcaagtt tgcccactcg
      661 attctgcagt gcttggatgc tttgcacaaa aacagaataa ttcactgtga ccttaagccc
      721 gagaacattt tgttaaagca gcagggtaga agcggtatta aagtaattga ttttggctcc
      781 agttgttacg agcatcagcg tgtctacacg tacatccagt cgcgttttta ccgggctcca
      841 gaagtgatcc ttggggccag gtatggcatg cccattgata tgtggagcct gggctgcatt
      901 ttagcagagc tcctgacggg ttaccccctc ttgcctgggg aagatgaagg ggaccagctg
      961 gcctgtatga ttgaactgtt gggcatgccc tcacagaaac tgctggatgc atccaaacga
     1021 gccaaa
//
