LOCUS       sequence-86636-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; 3' entry vector for 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: p3E_GGWDest+
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..150
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(151..167)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     189..206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    323..344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(359..364,365..382,383..389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    397..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     402..424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     421..437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             433..816
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        455..473
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     455..472
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      567..576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      571..580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(611..629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(636..653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(636..652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atacgactca ctatagggga tatcgcggcc gcgttaacgc taccatggag ctccaaataa
       61 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt
      121 ttataatgcc aactttgtat aataaagttg gataccgtcg acctcgagcc ttaagagacc
      181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      301 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      361 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      421 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
      481 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa
      541 ttcactagtg atatcgaatt cccgcggccg ccatggcggc cgggagcatg cgacgtcggg
      601 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
      661 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      721 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      781 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc gggtctcgcc atgg
//
