LOCUS       sequence-86637-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FBXL11A-c316
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             526..543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(596..614)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      610..657
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      694..1073
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(781..800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     991..1012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctctn aataattttg tttaacttta agaaggagat atacatatgc accatcatca
       61 tcatcattct tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgagacg
      121 agtgcgatgt cgaaaatgca aagcctgtgt gcaaggagag tgtggtgttt gccactactg
      181 cagagacatg aagaagtttg gggggcctgg acgcatgaag cagtcctgtg tcctccgaca
      241 gtgcttggca cccagactgc ctcactcagt cacatgttcc ctctgtggag aggtggatca
      301 gaatgaagag acacaagact ttgagaagaa actcatggaa tgctgtatct gcaatgagat
      361 tgttcatcct ggctgcctcc agatggacgg agaggggttg cttaacgaag aattgccaaa
      421 ttgctgggaa tgtccaaagt gctaccagga ggacagctcg tagcagtaaa ggtggatacg
      481 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      541 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      601 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      661 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1021 tcaaccctat ctcggtctat tcttttgatt ataaggaatt ttgccgattt cggcctattg
     1081 gttaaaaatg agctgattaa caaaa
//
