LOCUS       sequence-86640-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEK1A-c011
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggag aagtatgtta
      121 gactacagaa gattggagaa ggttcatttg gaaaagccat tcttgttaaa tctacagaag
      181 atggcagaca gtatgttatc aaggaaatta acatctcaag aatgtccagt aaagaaagag
      241 aagaatcaag gagagaagtt gcagtattgg caaacatgaa gcatccaaat attgtccagt
      301 atagagaatc atttgaagaa aatggctctc tctacatagt aatggattac tgtgagggag
      361 gggatctgtt taagcgaata aatgctcaga aaggcgtttt gtttcaagag gatcagattt
      421 tggactggtt tgtacagata tgtttggccc tgaaacatgt acatgataga aaaattcttc
      481 atcgagacat taaatctcag aacatatttt taactaaaga tggaacagta caacttggag
      541 attttggaat tgctagagtt cttaatagta ctgtagagct ggctcgagct tgcataggga
      601 ccccatacta cttgtcacct gaaatctgtg aaaacaaacc ttacaataat aaaagtgaca
      661 tttgggctct ggggtgtgtc ctttatgagc tgtgtacact taaacatgct tttgaagctg
      721 gcagtatgaa aaacctggta ctgaagataa tatctggatc ttttccacct gtgtctttgc
      781 attattccta tgatctccgc agtttggtgt ctcagttatt taaaagaaat cctagggata
      841 gaccatcagt caactccata ttggagaaag gttttatagc caaacgcatt gaaaagtttc
      901 tctctcctca gcttattgca gaagaatttt gtctaaaaac attttcgaag tttggatcac
      961 agcctatacc agctaaaaga ccagcttcag gacaaaactc gatttctgtt atgcctgctc
     1021 agaaaattac aaagcctgcc gctaaatatg gaataccttt agcatataag aaatatggag
     1081 ataaaaaatg ac
//
