LOCUS       sequence-86642-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SNX27A-c006
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             501..518
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(571..589)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      585..632
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(756..775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggatta cacagaaaag
      121 caagcagtgc ccatatcggt ccccagatac aaacatgtgg agcagaatgg tgagaagttt
      181 gtggtatata atgtttacat ggcagggagg cagctgtgtt ctaagcggta ccgggagttt
      241 gctatcctac accagaacct gaagagagag tttgccaact ttacatttcc tcgactccca
      301 gggaagtggc cattttcatt atcagaacaa caattagatg cccgacgtcg gggattggaa
      361 gaatatctag aaaaagtgtg ttcaatacga gtaattggtg agagtgacat catgcaggaa
      421 ttcctatcag aatcctgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      481 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      541 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      601 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      661 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      841 ccgatttagt gctttacggc acctcgaccc c
//
