LOCUS       sequence-86644-e        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TSTA3A-c013
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gcggattcta gtgacagggg
      121 gctctgggct ggtaggcaaa gccatccaga aggtggtagc agatggagct ggacttcctg
      181 gagaggactg ggtgtttgtc tcctctaaag acgccgatct cacggataca gcacagaccc
      241 gcgccctgtt tgagaaggtc caacccacac acgtcatcca tcttgctgca atggtggggg
      301 gcctgttccg gaatatcaaa tacaatttgg acttctggag gaaaaacgtg cacatgaacg
      361 acaacgtcct gcactcggcc tttgaggtgg gcgcccgcaa ggtggtgtcc tgcctgtcca
      421 cctgtatctt ccctgacaag acgacctacc cgatagatga gaccatgatc cacaatgggc
      481 ctccccacaa cagcaatttt gggtactcgt atgccaagag gatgatcgac gtgcagaaca
      541 gggcctactt ccagcagtac ggctgcacct tcaccgctgt catccccacc aacgtcttcg
      601 ggccccacga caacttcaac atcgaggatg gccacgtgct gcctggcctc atccacaagg
      661 tgcacctggc caagagcagc ggctcggccc tgacggtgtg gggtacaggg aatccgcgga
      721 ggcagttcat atactcgctg gacctggccc agctctttat ctgggtcctg cgggagtaca
      781 atgaagtgga gcccatcatc ctctccgtgg gcgaggaaga tgaggtctcc atcaaggagg
      841 cagccgaggc ggtggtggag gccatggact tccatgggga agtcaccttt gatacaacca
      901 agtcggatgg gcagtttaag aagacagcca gtaacagcaa gctgaggacc tacctgcccg
      961 acttccggtt cacacccttc aagcaggcgg tgaaggagac ctgtgcttgg ttcactgaca
     1021 actacgagca ggcccggaag tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
     1081 cgacaagctt gcggccgc
//
