LOCUS       sequence-8665-ex        3761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 N215-273C
FEATURES             Location/Qualifiers
     source          1..3761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2227..2298
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2316..2349
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2361..2381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2366..2695
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2546..2681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2705..3304
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2705..2724)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3201..3221
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             3305..3353
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgg cggcgctgcc cggcgcggtc
      901 cccaggatga tgagacccgg cccggggcag aactacccgc gcaccggctt ccccctggaa
      961 gtgtccaccc ctcttggcca aggccgggtc aatcagcttg gtggggtctt catcaatggt
     1021 cgacccctgc cgaaccacat ccgtcacaag atagtggaaa tggcccacca tggcatccgg
     1081 ccctgcgtca tctcccgtca gctccgtgtt tctcatggtt gtgtctccaa gattctgtgc
     1141 cgatatcagg agactgggtc catccggccc ggggctatcg gaggcagcaa gcccagacag
     1201 gtggcgactc cggatgtgga gaaaaagatt gaggagtata agagagagaa ccccgggatg
     1261 ttcagctggg aaatccggga ccggctgctg aaggacggtc actgcgaccg aagcacggtg
     1321 ccctcagtga gttcgattag ccgagtgctc agaatcaagt tcgggaagaa agaggacgac
     1381 gaggaaggag acaagaaaga agaagatggc gagaagaaag ccaaacacag catcgatggc
     1441 atcctgggcg acaaagggaa ccgtctggat gagggctcag atgtggaatc agaacccgac
     1501 ctccccctga agcgcaagca ggaattccag gcaggagcta accagctggc cgccttcaac
     1561 caccttctgc cgggaggttt cccacccacc ggcatgccca cgctgccacc ctaccagctg
     1621 ccggactcta cctaccccac caccaccatc tcccaagatg ggggcagcac agtacacagg
     1681 ccccagcccc ttccgccatc aaccatgcat cagggtgggc tggctgcggc cgctgcagca
     1741 gcagacacca gctctgccta cggagcccgc cacagcttct ccagctactc tgacagcttc
     1801 atgaaccctg gggctccctc caaccacatg aaccctgtca gcaatggcct gtctcctcag
     1861 gtcatgagca tccttagcaa cccgagtgcc gtgcctccac agccccaggc cgacttctcc
     1921 atctccccgc tgcatggagg cctggactcg gcttcctcca tctcagccag ctgcagccaa
     1981 cgggccgact ccatcaagcc aggagacagc ttgcccacgt cccagtctta ctgcccaccc
     2041 acctacagca ccactggcta cagtgtggac cctgtggctg gctaccagta cagccagtat
     2101 ggccaaactg ctgttgatta cctggccaaa aacgtgagcc tgtccacaca gcgccgtatg
     2161 aagcttgggg aacactccgc tgtgctggga cttcttcctg tggaaacggg acaagcctac
     2221 ctcgaggact acaaggatga cgatgacaag gattacaaag acgacgatga taaggactat
     2281 aaggatgatg acgacaaata atagtctaga gatccataac ttcgtataat gtatgctata
     2341 cgaagttatc tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2461 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2521 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2581 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2641 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     2701 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     2761 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     2821 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     2881 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     2941 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3001 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3061 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3121 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3181 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3241 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3301 ctgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcgata
     3361 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     3421 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaaata cataactgag
     3481 aatagagaag ttcagatcag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     3541 atatctgtgg taagcagttc ctgcccggct cagggccaag aacagatgga acagctgaat
     3601 atgggccaac agaatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     3661 tggtccccaa atgcggtcca gccctcagca gtttctagag aacatcagat gtttccaggg
     3721 tgcccaagac tgaatgacct gtgctatgac tacatcagag c
//
