LOCUS       sequence-86652-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SNX3A-c018
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(637..655)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      651..698
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(822..841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgccccc cagcaacttc
      121 ctcgagatcg atgtgagcaa cccgcaaacg gtgggggtcg gccggggccg cttcaccact
      181 tacgaaatca gggtcaagac aaatcttcct attttcaagc tgaaagaatc tactgttaga
      241 agaagataca gtgactttga atggctgcga agtgaattag aaagagagag caaggtcgta
      301 gttcccccgc tccctgggaa agcgtttttg cgtcagcttc cttttagagg agatgatgga
      361 atatttgatg acaattttat tgaggaaaga aaacaagggc tggagcagtt tataaacaag
      421 gtcgctggtc atcctctggc acagaacgaa cgttgtcttc acatgttttt acaagatgaa
      481 ataatagata aaagctatac ttgacagtaa aggtggatac ggatccgaat tcgagctccg
      541 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      661 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      721 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      781 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      841 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      901 agggttccga
//
