LOCUS       sequence-86653-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7-dependent expression of the type-I agrB and agrD genes of S. 
            aureus RN4220.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Marchand N, Collins CH
  TITLE     Peptide-based communication system enables Escherichia coli to 
            Bacillus megaterium interspecies signaling.
  JOURNAL   Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975.
            Epub 2013 Jul 5.
  PUBMED    23775238
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24975";
            journalName: "Biotechnol Bioeng"; date: "2013-11"; volume: "110"; 
            issue: "11"; pages: "3003-12"
COMMENT     Sequence Label: pT7-agrBD-I
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg aattcaggag cccttcacca tgaattattt tgataataaa attgaccagt
      181 ttgccacgta tcttcaaaag agaaataact tagatcatat tcaatttttg caagtacgat
      241 tagggatgca ggtcttagct aaaaatatag gtaaattaat tgttatgtat actattgcct
      301 atattttaaa catttttctg tttacgttaa ttacgaattt aacattttat ttaataagaa
      361 gacatgcaca tggtgcacat gcaccttctt ctttttggtg ttatgtagaa agtattatac
      421 tatttatact tttaccttta gtaatagtaa attttcatat taacttttta attatgatta
      481 ttttaacagt tatttcttta ggtgtaatct cagtatatgc tcctgcagca actaaaaaga
      541 agcccattcc tgtgcgactt attaaacgaa aaaaatatta tgcgattatt gttagtttaa
      601 cccttttcat tatcacactt atcatcaaag agccatttgc ccaattcatt caattaggca
      661 tcataataga agctattaca ttattaccta ttttctttat taaggaggac ttaaaatgaa
      721 tacattattt aacttatttt ttgattttat tactgggatt ttaaaaaaca ttggtaacat
      781 cgcagcttat agtacttgtg acttcataat ggatgaagtt gaagtaccaa aagaattaac
      841 acaattacac gaataaaagg gcgagctcaa ttcg
//
