LOCUS       sequence-86655-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FEVA-c012
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             457..474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(527..545)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      541..588
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      625..1005
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(712..731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     922..943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gaaaggcagc ggacagatcc agctgtggca
      121 gtttctgctg gagctgctgg ctgaccgcgc gaacgccggc tgcatcgcgt gggagggcgg
      181 tcacggcgag ttcaagctca cggacccgga cgaggtggcg cggcggtggg gcgagcgcaa
      241 gagcaagccc aacatgaact acgacaagct gagccgcgcc ctgcgctact actacgacaa
      301 gaacatcatg agcaaggtgc atggcaagcg ctacgcctac cgcttcgact tccagggcct
      361 ggcgcaggcc tgccagccgc cgcccgcgca ctgacagtaa aggtggatac ggatccgaat
      421 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      541 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      601 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      841 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      961 tctcggtcta ttcttttgat ttataaggga ttttgccaat ttcggc
//
