LOCUS       sequence-86657-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MYO5BA-c002
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgctc aacaggcagg
      121 tcacggtcca gcggaaagag aaggatttcc agggcatgct ggagtaccac aaagaggacg
      181 aggccctcct catccggaac ctggtgacag acttgaagcc ccagatgctg tcgggcacag
      241 tgccctgtct ccccgcctac atcctctaca tgtgcatccg gcacgcggac tacaccaacg
      301 acgatctcaa ggtgcactcc ctgctgacct ccaccatcaa cggcattaag aaagtcctga
      361 aaaagcacaa tgatgacttt gagatgacgt cattctggtt atccaacacc tgccgccttc
      421 ttcactgtct gaagcagtac agcggggatg agggcttcat gactcagaac actgcaaagc
      481 agaatgaaca ctgtcttaag aattttgacc tcaccgaata ccgtcaggtg ctgagtgacc
      541 tttccattca gatctaccag cagctcatta aaattgccga gggcgtgtta cagccgatga
      601 tagtttctgc catgttggaa aatgagagca ttcagggtct atctggtgtg aagcccaccg
      661 gctaccggaa gcgctcctcc agcatggcag atggggataa ctcatactgc ctggaagcta
      721 tcatccgcca gatgaatgcc tttcatacag tcatgtgtga ccagggcttg gaccctgaga
      781 tcatcctgca ggtattcaaa cagctcttct acatgatcaa cgcagtgact cttaacaacc
      841 tgctcttgcg gaaggacgtc tgctcttgga gcacaggcat gcaactcagg tacaatataa
      901 gtcagcttga ggagtggctt cggggaagaa accttcacca gagtggagca gttcagacca
      961 tggaacctct gatccaagca gcccagctcc tgcaattaaa gaagaaaacc caggaggacg
     1021 cagaggctat ctgctccctg tgtacctccc tcagcaccca gcagattgtc aaaattttaa
     1081 acctttatac tcccctgaat gaatttgaa
//
