LOCUS       sequence-86658-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SNX14B-c101
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             602..619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(857..876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gactccccga aaccttgctg
      121 catggaaaat tagcattcca tatgtagact tttttgagga tccctcctct gaaaggaagg
      181 agaaaaaaga aagaattcct gtgttttgta ttgatgttga aagaaatgat agaagagcag
      241 ttggacacga gcctgaacat tggtctgtct atagaagata tcttgaattc tatgtacttg
      301 aatcaaaact aacagaattt catggtgcat ttcctgatgc ccagcttcct tctaagagga
      361 tcattggccc caaaaattat gaattcttaa agtcaaagag ggaagagttc caagaatatc
      421 tacagaaact tctgcagcat ccagaactga gtaatagtca acttctggca gactttcttt
      481 cccctaatgg tggggaaaca caatttcttg ataagatact accagatgta aatctttgac
      541 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      601 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      901 tttccccgtc a
//
