LOCUS       sequence-86659-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SOCS4B-c002
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             663..680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(733..751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      747..794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(918..937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcttgt accagacctc
      121 cttcagatca ataacaaccc atgttactgg ggagtgatgg ataaatacgc agccgaagca
      181 ctactggaag gaaaaccaga gggtaccttt ttacttcgag actcagcaca ggaagactat
      241 ttattctctg ttagttttag acgctatagt cgttctcttc atgctagaat tgaacagtgg
      301 aatcacaact ttagctttga tgcacatgac ccctgtgtct tccattctcc tgacattact
      361 gggctcctag aacattataa ggacccaagc gcctgtatgt tctttgaacc acttctatcc
      421 actcccttaa ttcggacttt ccctttttcc ctgcagcata tatgcagaac agttatttgt
      481 aactgtacaa cttatgatgg catcgatgcc cttccaattc cttcttctat gaaattatat
      541 ctgaaggaat atcattataa atcaaaagtt agagtactca ggattgatgc accagaatga
      601 cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
      661 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      721 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      781 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      841 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      901 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
//
