LOCUS       sequence-86661-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASB9A-c009
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             817..837
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             838..855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             856..879
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             929..946
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ccttaagaag gagatatact atgtctgatt ggtctcctat gcatgaagct
       61 gcaatccacg gacatcagct gtctctgagg aacctcatca gccaggggtg ggctgtgaac
      121 atcatcacgg cagatcatgt ttccccactc catgaagcct gtcttggagg tcatctctct
      181 tgtgtgaaga ttttattaaa gcatggagct caggtgaatg gtgtgacagc agactggcac
      241 actccactgt ttaatgcttg tgtcagcggc agctgggatt gtgtgaattt gcttctgcag
      301 cacggagcca gcgttcaacc tgagagtgat ctggcatccc ccatccatga agctgctagg
      361 agaggccacg tggagtgtgt caactctctt atagcttatg ggggcaacat tgaccataag
      421 atcagccacc tgggcactcc actctatttg gcttgtgaaa accaacagag agcctgtgtc
      481 aagaagcttc tggagtcagg agcggacgtg aaccaaggga aaggtcagga ttccccactt
      541 catgcagtgg ccaggacagc cagtgaagag ctggcctgcc tgctcatgga ttttggagcg
      601 gacacccagg ccaagaatgc tgaaggcaaa cgtcctgtgg agctggtgcc tccagagagc
      661 cccttggccc agctcttctt ggagagagaa gggccccctt ctttgatgca gttatgccgc
      721 cttagaattc ggaagtgttt tggaatccag cagcatcata agataaccaa actcgtcctc
      781 ccagaggatc tgaaacagtt tctcctacat cttgcagaga acctctactt ccaatcgcac
      841 catcatcacc accatgatta caaggatgac gacgataagt gaggatccga attcgagctc
      901 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      961 taacaagccc gaaaggaagc tga
//
