LOCUS       sequence-86662-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human MEK1 from puromycin resistance retroviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEpuro-HA-MEK1
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..54
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gccggatccg atatcgccgc caccatgtac ccatacgatg ttccagatta cgctcccaag
       61 aagaagccga cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc
      121 agctctgcgg agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat
      181 gagcagcagc gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg
      241 aaggatgacg actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc
      301 aaggtctccc acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc
      361 aaacccgcaa tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct
      421 ccgtacatcg tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg
      481 gagcacatgg atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa
      541 caaattttag gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggaaaag
      601 cacaagatca tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag
      661 atcaagctct gtgactttgg ggtcagcggg cagctcatcg actccatggc caactccttc
      721 gtgggcacaa ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag
      781 tcagacatct ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc
      841 cctcctccag atgccaagga gctggagctg atgtttgggt gccaggtgga aggagatgcg
      901 gctgagaccc cacccaggcc aaggaccccc gggaggcccc ttagctcata cggaatggac
      961 agccgacctc ccatggcaat ttttgagttg ttggattaca tagtcaacga gcctcctcca
     1021 aaactgccca gtggagtgtt cagt
//
