LOCUS       sequence-86663-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP
  TITLE     Rab35 controls actin bundling by recruiting fascin as an effector 
            protein.
  JOURNAL   Science. 2009 Sep 4;325(5945):1250-4. doi: 10.1126/science.1174921.
  PUBMED    19729655
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-09-4- 4"; volume: "325"; issue: 
            "5945"; pages: "1250-4"
COMMENT     Sequence Label: pUAST 6xmyc Rab35 WT
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..88
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             137..166
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             176..205
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             278..307
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    320..344
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actctgaata gggaattggg aattatcgag gcctgtctag agaagctccg ccaccatgga
       61 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga
      121 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagca
      181 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga
      241 cttgaatgaa atggagagct tgggcgacct caccatggag caaaagctca tttctgaaga
      301 ggacttgaat caccggtata caagtttgta caaaaaagca ggcttaatgg cacgcggctt
      361 cgatcatcta ttcaagttgc taataatcgg cgatagcggc gtgggcaagt cgtcgctgct
      421 catccggttc tcggacgaca cattctcagg cagttacatc accaccatcg gcgtggactt
      481 taagatccgc accgtggaca tcgagggcat gcgcgtaaag ctgcagatct gggacacggc
      541 cgggcaggag cgcttccgga caatcaccag cacctactac cgcggcaccc acggcgtcat
      601 tgtcgtctac gacgtcacga acggcgaatc ctttgcgaac gtccgccgct ggctggagga
      661 gatccagaat aactgcgatg tggttaaaaa agtattagtg ggcaacaaga acgacgatcc
      721 ggacaggaag gtggtcatta ccgaagacgc gcaacggttt gcgaaacaaa tggacatcga
      781 gctattcgag acatctgcca aagacaacat aaacgtggag aacatgttct tgtcgattac
      841 gcgacaggtg ctcgatcaca agctgcgcac ctccc
//
