LOCUS       sequence-86664-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP1AA-c001
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(99..101,102..425)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             488..505
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(558..576)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      572..619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(743..762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggagtgcag gtggaaacca
      121 tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca
      181 ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta
      241 agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga
      301 gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc
      361 acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg
      421 aatgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      481 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      541 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      601 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      661 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      721 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      781 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      841 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      901 ccctgataga cggtttttcg ccctttgacg ttgga
//
