LOCUS       sequence-8667-ex        5832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Reiser J, Harmison G, Kluepfel-Stahl S, Brady RO, Karlsson S, 
            Schubert M
  TITLE     Transduction of nondividing cells using pseudotyped defective 
            high-titer HIV type 1 particles.
  JOURNAL   Proc Natl Acad Sci U S A. 1996 Dec 24. 93(26):15266-71.
  PUBMED    8986799
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1996 Dec 24. 93(26):15266-71."
COMMENT     Sequence Label: pLTR-G
FEATURES             Location/Qualifiers
     source          1..5832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(782..829,830..2317)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    2620..2741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2657..2676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2711..2730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2748..3203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2885..2906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3097..3116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3230..3334
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3336..3693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3336..3527
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3359..3379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3544..3679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3728..4522
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3728..4522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3782..3801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4392..4411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4710..4729)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4754..4801
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5130..5718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5619..5638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattac cggactcaga tctcgagacc tagaaaaaca tggagcaatc acaagtagca
       61 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg
      121 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
      181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
      241 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
      301 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
      361 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
      421 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca
      481 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
      541 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
      601 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt
      661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      721 ccactgctta agcctcaata aagcttctag agatccctcg acctcgagga attctgacac
      781 tatgaagtgc cttttgtact tagccttttt attcattggg gtgaattgca agttcaccat
      841 agtttttcca cacaaccaaa aaggaaactg gaaaaatgtt ccttctaatt accattattg
      901 cccgtcaagc tcagatttaa attggcataa tgacttaata ggcacagcct tacaagtcaa
      961 aatgcccaag agtcacaagg ctattcaagc agacggttgg atgtgtcatg cttccaaatg
     1021 ggtcactact tgtgatttcc gctggtatgg accgaagtat ataacacatt ccatccgatc
     1081 cttcactcca tctgtagaac aatgcaagga aagcattgaa caaacgaaac aaggaacttg
     1141 gctgaatcca ggcttccctc ctcaaagttg tggatatgca actgtgacgg atgccgaagc
     1201 agtgattgtc caggtgactc ctcaccatgt gctggttgat gaatacacag gagaatgggt
     1261 tgattcacag ttcatcaacg gaaaatgcag caattacata tgccccactg tccataactc
     1321 tacaacctgg cattctgact ataaggtcaa agggctatgt gattctaacc tcatttccat
     1381 ggacatcacc ttcttctcag aggacggaga gctatcatcc ctgggaaagg agggcacagg
     1441 gttcagaagt aactactttg cttatgaaac tggaggcaag gcctgcaaaa tgcaatactg
     1501 caagcattgg ggagtcagac tcccatcagg tgtctggttc gagatggctg ataaggatct
     1561 ctttgctgca gccagattcc ctgaatgccc agaagggtca agtatctctg ctccatctca
     1621 gacctcagtg gatgtaagtc taattcagga cgttgagagg atcttggatt attccctctg
     1681 ccaagaaacc tggagcaaaa tcagagcggg tcttccaatc tctccagtgg atctcagcta
     1741 tcttgctcct aaaaacccag gaaccggtcc tgctttcacc ataatcaatg gtaccctaaa
     1801 atactttgag accagataca tcagagtcga tattgctgct ccaatcctct caagaatggt
     1861 cggaatgatc agtggaacta ccacagaaag ggaactgtgg gatgactggg caccatatga
     1921 agacgtggaa attggaccca atggagttct gaggaccagt tcaggatata agtttccttt
     1981 atacatgatt ggacatggta tgttggactc cgatcttcat cttagctcaa aggctcaggt
     2041 gttcgaacat cctcacattc aagacgctgc ttcgcaactt cctgatgatg agagtttatt
     2101 ttttggtgat actgggctat ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag
     2161 ttggaaaagc tctattgcct cttttttctt tatcataggg ttaatcattg gactattctt
     2221 ggttctccga gttggtatcc atctttgcat taaattaaag cacaccaaga aaagacagat
     2281 ttatacagac atagagatga accgacttgg aaagtaactc aaatcctgca caacagattc
     2341 ttcatgtttg gaccaaatca acttgtgata ccatgctcaa agaggcctca attatatttg
     2401 agtttttaat ttttatgaaa aaaaaaaaaa aaaacggaat tcctcgaggg atcttccata
     2461 cctaccagtt ctgcgcctgc aggtcgcggc cgatccaccg gatctagata actgatcata
     2521 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2581 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     2761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3181 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3301 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3661 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3721 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3901 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     3961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4261 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4501 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4561 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4681 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4741 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4801 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4861 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4921 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     4981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5821 accgccatgc at
//
