LOCUS       sequence-86673-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AK5A-c001
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      347..356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             762..779
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(832..850)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      846..893
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1017..1036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgggagg tttcatggaa
      121 gatttgagaa agtgtaaaat tattttcata attggtggtc ctggctctgg caaaggcaca
      181 cagtgtgaaa agctggtgga aaaatatgga tttacacatc tctcaactgg cgagctcctg
      241 cgtgaggaac tggcatcaga atctgaaaga agcaaattga tcagagacat tatggaacgt
      301 ggagacctgg tgccctcagg catcgttttg gagctcctga aggaggccat ggtggccagc
      361 ctcggggaca ccaggggctt cctgattgac ggctatcctc gggaggtgaa gcaaggggaa
      421 gagttcggac gcaggattgg agacccacag ttggtgatct gtatggactg ctcggcagac
      481 accatgacca accgccttct ccaaatgagc cggagcagcc tgcctgtgga cgacaccacc
      541 aagaccatcg ccaagcgcct agaagcctac taccgagcgt ccatccccgt gatcgcctac
      601 tacgagacaa aaacacagct acacaagata aatgcagagg gaacaccaga ggacgttttt
      661 cttcaactct gcacagctat tgactctatt ttcttgtgac agtaaaggtg gatacggatc
      721 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1021 tttcgctttc ttccctcctt tctcgccacg ttcgccggct ttccccgtca
//
