LOCUS       sequence-86674-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Controls GFP expression from the P3 promoter cloned out of S. aureus
            RN4220 genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Marchand N, Collins CH
  TITLE     Peptide-based communication system enables Escherichia coli to 
            Bacillus megaterium interspecies signaling.
  JOURNAL   Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975.
            Epub 2013 Jul 5.
  PUBMED    23775238
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24975";
            journalName: "Biotechnol Bioeng"; date: "2013-11"; volume: "110"; 
            issue: "11"; pages: "3003-12"
COMMENT     Sequence Label: pP3-GFP
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(154..201)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(285..305)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgccagcaac gcggcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
       61 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
      121 caattcttgg accggatata gttcctcctt tagcaaaaaa cccctcaaga cccgtttaga
      181 ggccccaagg ggttatgcta gttaaccggt cgcgattagc cggcatgcgg ccgcgaattc
      241 attatttgta gggctcatcc atgccatgtg taatcccagc agcagttaca aactcaagaa
      301 ggaccatgtg gtcacgcttt tcgttgggat ctttcgaaag ggcagattgt gtcgacaggt
      361 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt
      421 ctgctagttg aacggatcca tcttcaatgt tgtggcgaat tttgaagtta gctttgattc
      481 cattcttttg tttgtctgcc gtgatgtata cattgtgtga gttatagttg tactcgagtt
      541 tgtgtccgag aatgtttcca tcttctttaa aatcaatacc ttttaactcg atacgattaa
      601 caagggtatc accttcaaac ttgacttcag cacgcgtctt gtagttcccg tcatctttga
      661 aagatatagt gcgttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgcc
      721 gtttcatatg atccggataa cgggaaaagc attgaacacc ataggtcaaa gtagtgacaa
      781 gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagctttc
      841 cgtatgtagc atcaccttca ccctctccac tgaca
//
