LOCUS       sequence-86679-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZBTB33A-c002
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             528..545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(598..616)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      612..659
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(783..802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggagag tagaaaactg
      121 atttctgcta cagacattca gtactctggc agtctgctga actccttgaa tgagcaacgt
      181 ggccatggac tcttctgtga tgttaccgtt attgtggaag accgaaaatt ccgggctcac
      241 aagaatattc tttcagcttc tagtacctac ttccatcagc tcttctctgt tgctgggcaa
      301 gttgttgaac tgagctttat aagagcagag atctttgcag aaattctcaa ttatatctat
      361 agttctaaaa ttgttcgtgt tagatcagat ttgcttgatg agttaattaa atcagggcag
      421 ttattaggag tgaaatttat agcagcgctt ggtgtcccat tgtgacagta aaggtggata
      481 cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
      541 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      601 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      661 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cgggttttcc
      961 cccctttgac gttggagtcc acgttcttta atag
//
