LOCUS       sequence-86680-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HTD2A-c022
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             605..622
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(675..693)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      689..736
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gctgccagtg ctgaccctgc
      121 agcactttca gcatatgcac atcaaagttg gagaccgcgc tgaacttagg agggccttca
      181 cacagactga tgtggctacc ttctcagaat taacagggga tgtcaatcct ttgcatttga
      241 atgaagactt tgcaaaacac accaagtttg gaaatacaat tgtacatgga gttttgatca
      301 acggacttat ctcagctctc ctaggaacta aaatgccagg gccaggctgt gtatttcttt
      361 cccaggaaat tagctttcca gcccctttat atattggaga agttgtttta gcttctgcag
      421 aagtgaaaaa gctgaagcgg ttcattgcta ttattgcagt gtcatgttct gtaatagaaa
      481 gtaaaaagac tgttatggaa ggctgggtta aagttatggt tccagaagct tccaaatcct
      541 gacagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact
      601 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      721 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      841 gccagcgccc tagcgcccgc tcctttcgct ttcttcccct tcctttctcg ccacgttcgc
      901 cggctttccc cgtcagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      961 cggcacctcg accccaaaaa ct
//
