LOCUS       sequence-86690-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ETV1A-c014
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             483..500
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(553..571)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      567..614
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(738..757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgggacc cacatcccaa
      121 cggcgaggat cacttcagct ctggcagttt ttggtagctc ttctggatga cccttcaaat
      181 tctcatttta ttgcctggac tggtcgaggc atggaattta aactgattga gcctgaagag
      241 gtggcccgac gttggggcat tcagaaaaac aggccagcta tgaactatga taaacttagc
      301 cgttcactcc gctattacta tgagaaagga attatgcaaa aggtggctgg agagagatat
      361 gtctacaagt ttgtgtgtga tccagaagcc cttttctcca tggccttttc agataattga
      421 cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
      481 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      541 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      601 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      661 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      721 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      781 ctttccccgt caagctctaa atcggggggc tccctttagg gttccgattt agtgctttac
      841 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgcccc
      901 tgatagacgg ttttttcgcc cttt
//
