LOCUS       sequence-86693-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SNX24A-c003
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(555..573)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      569..616
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(740..759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggaggtctac atcccgtcct
      121 ttcgctatga agagagcgac ctggagcggg gatacacggt gtttaagata gaagtgctaa
      181 tgaatggaag aaaacatttt gttgaaaaga gatacagcga atttcatgct ttgcacaaaa
      241 agcttaagaa atgtataaaa actccagaaa tcccttctaa acatgttagg aactgggtcc
      301 ccaaagtctt ggaacagcga cgacaaggct tggaaacata cttacaggct gtcattttag
      361 aaaatgaaga acttcccaaa ctgtttcttg atttcctaaa tgtgcgacac ttgccctctt
      421 gacagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact
      481 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      541 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      601 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      661 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      721 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      781 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      841 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      901 tgatagacgg tttttcgccc ttttgacgtt ggagtccacg ttctttaata gtggactctg
      961 gttccaactg gaacaacact caaccctatc tcgg
//
