LOCUS       sequence-86695-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BIN2A-c011
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             872..889
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggcctcttc gccaagcagg
      121 tgcagaagaa gtttagcagg gcccaggaga aggtgctgca gaaattgggg aaagctgtag
      181 aaaccaaaga tgaacgattt gaacaaagcg ctagcaactt ctaccaacaa caggcagaag
      241 gccacaagct gtacaaggac ctgaagaact tccttagtgc agtcaaagtg atgcatgaaa
      301 gttcaaaaag agtgtcagaa accctgcagg agatctacag cagcgagtgg gacggtcatg
      361 aggagctgaa ggccatcgta tggaataatg atctcctttg ggaagactac gaggagaaac
      421 tggctgacca ggctgtaagg accatggaaa tctatgttgc ccagttcagt gaaattaagg
      481 agagaattgc caagcggggt cggaaactcg tggactatga cagtgcccga caccacctgg
      541 aggcagtgca gaatgccaag aagaaagatg aggccaagac tgccaaggca gaggaagagt
      601 tcaacaaagc ccagactgtg tttgaagatc tgaaccaaga actactagag gagctgccta
      661 ttctttataa tagtcgtatt ggctgctatg tgaccatctt ccaaaacatt tccaacttga
      721 gggatgtctt ctacagggaa atgagcaagc tgaaccacaa tctctacgag gtgatgagca
      781 aactggagag gcaacattcc aataaatgac agtaaagttg gatacggatc cgaattcgag
      841 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      901 tgctaacaaa gcccgaaagg aagctga
//
