LOCUS       sequence-86699-e        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAT2AA-c001
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gaacggacag ctcaacggct
      121 tccatgaggc gttcatcgag gagggcacat tccttttcac ctcagagtcg gtcggggaag
      181 gccacccaga taagatttgt gaccaaatca gtgatgctgt ccttgatgcc caccttcagc
      241 aggatcctga tgccaaagta gcttgtgaaa ctgttgctaa aactggaatg atccttcttg
      301 ctggggaaat tacatccaga gctgctgttg actaccagaa agtggttcgt gaagctgtta
      361 aacacattgg atatgatgat tcttccaaag gttttgacta caagacttgt aacgtgctgg
      421 tagccttgga gcaacagtca ccagatattg ctcaaggtgt tcatcttgac agaaatgaag
      481 aagacattgg tgctggagac cagggcttaa tgtttggcta tgccactgat gaaactgagg
      541 agtgtatgcc tttaaccatt gtcttggcac acaagctaaa tgccaaactg gcagaactac
      601 gccgtaatgg cactttgcct tggttacgcc ctgattctaa aactcaagtt actgtgcagt
      661 atatgcagga tcgaggtgct gtgcttccca tcagagtcca cacaattgtt atatctgttc
      721 agcatgatga agaggtttgt cttgatgaaa tgagggatgc cctaaaggag aaagtcatca
      781 aagcagttgt gcctgcgaaa taccttgatg aggatacaat ctaccaccta cagccaagtg
      841 gcagatttgt tattggtggg cctcagggtg atgctggttt gactggacgc aaaatcattg
      901 tggacactta tggcggttgg ggtgctcatg gaggaggtgc cttttcagga aaggattata
      961 ccaaggtcga ccgttcagct gcttatgctg ctcgttgggt ggcaaaatcc cttgttaaag
     1021 gaggtctgtg ccggagggtt cttgttcagg tctcttatgc tattggagtt tctcatccat
     1081 tatctatctc cattttcc
//
