LOCUS       sequence-86702-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag tagged human oncogenic BRAFV600E from bleomycin 
            resistance retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEbleo-Flag-BRAFV600E
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccggccggat cccagtgtgg tggtacgtag gaattcgcca gcacagtggt cgacgccgcc
       61 accatggatt ataaagatga cgacgataag gcggcgctga gcggtggcgg tggtggcggc
      121 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc
      181 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa
      241 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag
      301 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca
      361 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt
      421 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta
      481 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca
      541 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca
      601 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc
      661 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac
      721 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca
      781 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt
      841 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt
      901 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc
      961 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc
     1021 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc
     1081 accagtccgt ct
//
