LOCUS       sequence-8671-ex        6504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ham TS, Lee SK, Keasling JD, Arkin AP
  TITLE     A tightly regulated inducible expression system utilizing the fim 
            inversion recombination switch.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):1-4.
  PUBMED    16534780
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):1-4."
COMMENT     Sequence Label: pFIP
FEATURES             Location/Qualifiers
     source          1..6504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     1979..2001
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1994..2010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2020..2039
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2020..2038
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2079..2165
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2260..2346
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2441..2527
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2622..2708
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(join(2865..2870,2871..2887,2888..2893))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(3286..3303)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3456..3542
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3634..3661
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3680..3771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3772..3840,3841..4632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3990..4009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4674..5129
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4761..4780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5240..5828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5729..5748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5982..5999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6014..6154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6080..6099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6240..6262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6401..6420
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggga caggatatgt
     1321 gagtaaacgt cgttatctta ccggtaaaga agttcaggcc atgatgcagg cggtttgtta
     1381 cggggcaacg ggagccagag attattgtct tattctgttg gcatatcggc atgggatgcg
     1441 tattagtgaa ctgcttgatc tgcattatca ggaccttgac cttaatgaag gtagaataaa
     1501 tattcgccga ctgaagaacg gattttctac cgttcacccg ttacgttttg atgagcgtga
     1561 agccgtggaa cgctggaccc aggaacgtgc taactggaaa ggcgctgacc ggactgacgc
     1621 tatatttatt tctcgccgcg ggagtcggct ttctcgccag caggcctatc gcattattcg
     1681 cgatgccggt attgaagctg gaaccgtaac gcagactcat cctcatatgt taaggcatgc
     1741 ttgcggttat gaattggcgg agcgtggtgc agatactcgt ttaattcagg attatctcgg
     1801 gcatcgaaat attcgccata ctgtgcgtta taccgccagt aatgctgctc gttttgccgg
     1861 attatgggaa agaaataatc tcataaacga aaaattaaaa agagaagagg tttgatttaa
     1921 cttattgata ataaaggagc tcctgcaagg cgattaagtt gggtaacgcc agggttttcc
     1981 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     2041 gaattggagc tggggccgca attcccaatt ccaggcatca aataaaacga aaggctcagt
     2101 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     2161 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     2221 gacgcccgcc ataaactgcc aggaattaat tccaggcatc aaataaaacg aaaggctcag
     2281 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     2341 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
     2401 ggacgcccgc cataaactgc caggaattaa ttccaggcat caaataaaac gaaaggctca
     2461 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     2521 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     2581 aggacgcccg ccataaactg ccaggaatta attccaggca tcaaataaaa cgaaaggctc
     2641 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     2701 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     2761 caggacgccc gccataaact gccaggaatt ggggatcgga agcttctgcc aacactgcac
     2821 agttttcccc caaaagatga aacatttggg gccaaactgt ccatattata cgagccggat
     2881 gattaattgt caaccataaa aatattaaaa aacgacaaaa agcatctaac tgtttgatat
     2941 gtaaattatt tctattgtaa attaatttca catcacctcc gctatatgta aagctaacgt
     3001 ttctgtggct cgacgcatct tcctcattct tctctccaaa aaccacctca tgcaatataa
     3061 acatctataa ataaagataa caatagaata ttaagccaac aaataaactg aaaaagtttg
     3121 tccgcgatgc tttcctctat gagtcaaaat ggccccaatt gtcttgtatt tatttgtttt
     3181 tttaacttta ttatcaataa gttaaatcgg tacccgggga tcctctagag tcgacctgca
     3241 ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
     3301 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     3361 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     3421 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     3481 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     3541 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     3601 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     3661 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3841 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3901 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3961 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     4021 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4081 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4141 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4201 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4261 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     4321 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4381 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4441 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4501 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4561 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4621 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
     4681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4861 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4981 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     5041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5101 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata
     5161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6001 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6061 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     6121 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     6181 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6241 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcaagg
     6301 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     6361 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     6421 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     6481 tctgctcatg tttgacagct tatc
//
