LOCUS       sequence-86718-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 
            (498–1344 with S311DT315D) from puromycin resistance retroviral 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEpuro-HA-MEK1-MEK5DD
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccggccggat ccgaatatcg ccgccaccat gtacccatac gatgttccag attacgctcc
       61 caagaagaag ccgacgccca tccagctgaa cccggccccc gacggctctg cagttaacgg
      121 gaccagctct gcggagacca acttggaggc cttgcagaag aagctggagg agctagagct
      181 tgatgagcag cagcgaaagc gccttgaggc ctttcttacc cagaagcaga aggtgggaga
      241 actgaaggat gacgacatac gatatcggga cactcttggt catggcaacg gaggcacagt
      301 ctacaaagca tatcatgtcc cgagtgggaa aatattagct gtaaaggtca tactactaga
      361 tattacactg gaacttcaga agcaaattat gtctgaattg gaaattcttt ataagtgcga
      421 ttcatcatat atcattggat tttatggagc attttttgta gaaaacagga tttcaatatg
      481 tacagaattc atggatgggg gatctttgga tgtatatagg aaaatgccag aacatgtcct
      541 tggaagaatt gcagtagcag ttgttaaagg ccttacttat ttgtggagtt taaagatttt
      601 acatagagac gtgaagccct ccaatatgct agtaaacaca agaggacagg ttaagctgtg
      661 tgattttgga gttagcactc agctggtgaa tgatatagcc aaggactatg ttggaacaaa
      721 tgcttatatg gcgcctgaaa ggatttcagg ggagcagtat ggaattcatt ctgatgtctg
      781 gagcttagga atctctttta tggagcttgc tcttgggagg tttccatatc ctcagattca
      841 gaaaaaccag ggatctttaa tgcctctcca gcttctgcag tgcattgttg atgaggattc
      901 gcccgtcctt ccagttggag agttctcgga gccatttgta catttcatca ctcagtgtat
      961 gcgaaaacag ccaaaagaaa ggccagcacc tgaagaattg atgggccacc cgttcatcgt
     1021 gcagttcaat gatggaaatg ccgccgtggt gtccatgtgg gtgtgccggg cgctgga
//
