LOCUS       sequence-8672-ex        6601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Pfeiffer BD, Jenett A, Hammonds AS, Ngo TT, Misra S, Murphy C, 
            Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, 
            Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM.
  TITLE     Tools for Neuroanatomy and Neurogenetics in Drosophila
  JOURNAL   Tools for neuroanatomy and neurogenetics in Drosophila
  PUBMED    18621688
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tools for 
            neuroanatomy and neurogenetics in Drosophila"
COMMENT     Sequence Label: pBDP
FEATURES             Location/Qualifiers
     source          1..6601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(join(1124..1738,1809..1940,2144..2459,2521..3175,
                     3250..3523,3925..3996))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4448..4466
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4691..4760
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(4847..5435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4927..4946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5606..6397,6398..6466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6229..6248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6467..6571)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta aacggccggc cagatctggt accggatccc cgcgcggccg
      121 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
      181 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
      241 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
      301 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggaggc gcgccctagt
      361 tccagtgaaa tccaagcatt ttctaaatta aatgtattct tattattata gttgttattt
      421 ttgatatata taaacaacac tattatgccc accatttttt tgagatgcat ctacacaagg
      481 aacaaacact ggatgtcact ttcagttcaa attgtaacgc taatcactcc gaacaggtca
      541 caaaaaatta ccttaaaaag tcataatatt aaattagaat aaatatagct gtgagggaaa
      601 tatatacaaa tatattggag caaataaatt gtacatacaa atatttatta ctaatttcta
      661 ttgagacgaa atgaaccact cggaaccatt tgagcgaacc gaatcgcgcg gaactaacga
      721 cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca
      781 gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat
      841 ttgaaaagta tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt
      901 tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt
      961 gtggggatgt tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc
     1021 acaaatataa ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa
     1081 tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1141 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1201 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1261 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1321 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1381 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1441 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1501 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1561 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1621 gtggcactgt gagaaaaagc ggcaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1681 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1741 aaatcgaatc gattcattag aaagttagta aattattgaa atgcaaatgt attctaaaca
     1801 tgacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     1861 atggctccgt tgatattcat cacacccact tgcgtgagtt gttggcccaa aaagatgagg
     1921 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcgc
     1981 gagttaaatg aaatttattt atcttctgca aaactataaa ctatacatct cattgaaaaa
     2041 aactaagaag ggtgtggaat caggcaattc tatctaaaat ctagcgaatt tgtttccaag
     2101 aattgtaagc gttatatcat ttgtttccac tggaaccact caccgttgtc tgaataagtc
     2161 gcacttttac gaggagtggt tccttgagca ccgacagcca ggatcgccac aggaccgccc
     2221 ggaactgcat gaaccaggtg gccttgtagg tgtacccatt ctccggctgc tccagtggct
     2281 tctccagatt tttggtggcc aacaactgct ccatatcccg ggctactttg ctaatggcaa
     2341 aattgtcgca tatcttggcg atccgatcac gggactcgat ctcccgtccg ggcacaacgg
     2401 ccaacacctg tacgtaaaag tccgccggat tgtagttggt aggacactgg gcacccacgc
     2461 tggataggag ttgagatgtt atgtaatact agataccctt aataaacaca tcgaactcac
     2521 taggaaaaga agtcgacggc ttcgctggga gtgcccaaga aagctaccct gccctcggcc
     2581 atcagaagga tcttgtcaaa gagctcaaac agctcggaag acggctgatg aatggtcagg
     2641 atgacggtct tgcccttctg cgacagcttc ttcagcacct ggacgacgct gtgggcggta
     2701 aaggagtcca gtccggaggt gggctcatcg cagatcagaa gcggcggatc ggttagagcc
     2761 tcggaggcga atgccagacg cttcctttct ccgccggaca gacctttcac cctgccgggc
     2821 acaccgatga tcgtgtgctg acatttgctg agcgaaagct cctggatcac ctgatccacg
     2881 cgggccactc gctgccgata ggtcagatgt cgtggcatcc gcaccatggc ttggaaaatc
     2941 aggtgttccc tggccgttag ggagccgata aagaggtcat cctgctggac ataggcgcac
     3001 ctggcctgca tctccttggc gtccacaggt tggccattga gcagtcgcat cccggatggc
     3061 gatacttgga tgccctgcgg cgatcgaaag gcaagggcat tcagcagggt cgtctttccg
     3121 gcaccggaac tgcccatcac ggccaaaagt tcgcccggat aggccacgcc gcaaactgag
     3181 tttcaaattg gtaattggac cctttattaa gatttcacac agatcagccg actgcgaata
     3241 gaaactcacc gttcttgagc aaatgtttcc tgggcgccgg tatgtgtcgc tcgttgcaga
     3301 atagtccgcg tgtccggttg accagctgcc gccatccgga gcccggctga ttgaccgccc
     3361 caaagatgtc catattgtgc caggcatagg tgaggttctc ggctagttgg ccgctccctg
     3421 aaccggagtc ctccggcgga ctgggtggcc ggagcgtgcc gtagtttttg gcctgcccga
     3481 agccctggtt aatgcagctc tgcgaagccg ctccgctgtc accctgcaat gataggggat
     3541 ctcaaatatc aactactagc gttatgctca tctaaccccg aacaaaaagt accccgaagt
     3601 atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat cagcttaaaa
     3661 tatctggttg ttatattttt tgtaaaaaag aatatagtcg aaaatgaatg cctttagatg
     3721 tcttgatcat gatatgatct caaaaattgt cttatatagc gagaacagct accagaataa
     3781 tctgtttcgt gtcactattt gtttgtgcaa ttgcggtttg ggatttttgt gggtcgcagt
     3841 tctcacgccg catacaattt gatgttgcaa tcgcagttcc tatagatcaa gtgaacttaa
     3901 gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt gtttgctgcc
     3961 tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc acactttccc
     4021 ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa caaaaataag
     4081 aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa ccgcttggag
     4141 cttcgtcacg aaaccgctga caaaatgcaa ctgaaggcgg acattgacgc tacgtaacgc
     4201 tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg atatttctgt
     4261 ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg agtgagaggt
     4321 aatcgaaaga acctggtaca tcaaataccc ttggatcgaa gtaaatttaa aactgatcag
     4381 ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg ttttttttat ctactttccg
     4441 caaaaatggg ttttattaac ttacatacat actaggcgcg cccatatgtt cggcttgtcg
     4501 acatgcccgc cgtgaccgtc gagaacccgc tgacgctgcc ccgcgtatcc gcacccgccg
     4561 acgccgtcgc acgtcccgtg ctcaccgtga ccaccgcgcc cagcggtttc gagggcgagg
     4621 gcttcccggt gcgccgcgcg ttcgccggga tcaactaccg ccacctcgac ccgttcatca
     4681 tgatggacca gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc
     4741 acccgcaccg cggcttcgag accgtgacct acatcgtcga cggtaacatg tgagcaaaag
     4801 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4861 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4921 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4981 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5041 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5101 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5161 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5221 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5281 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5341 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5401 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5461 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5521 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5581 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5641 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5701 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5761 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5821 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5881 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5941 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6001 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6061 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6121 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6181 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6241 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6301 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6361 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6421 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6481 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6541 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgagt
     6601 c
//
