LOCUS       sequence-86727-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human MEK1 M187A/H188A/M230A/H239A from 
            puromycin resistance retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEpuro-HA-MEK1 CBM
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..57
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccggccggat ccgatatcgc cgccaccatg tacccatacg atgttccaga ttacgctccc
       61 aagaagaagc cgacgcccat ccagctggac ccggcccccg acggctctgc agttaacggg
      121 accagctctg cggagaccaa cttggaggcc ttgcagaaga agctggagga gctagagctt
      181 gatgagcagc agcgaaagcg ccttgaggcc tttcttaccc agaagcagaa ggtgggagaa
      241 ctgaaggatg acgactttga gaagatcagt gagctggggg ctggcaatgg cggtgtggtg
      301 ttcaaggtct cccacaagcc ttctggcctg gtcatggcca gaaagctaat tcatctggag
      361 atcaaacccg caatccggaa ccagatcata agggagctgc aggttctgca tgagtgcaac
      421 tctccgtaca tcgtgggctt ctatggtgcg ttctacagcg atggcgagat cagtatctgc
      481 atggagcaca tggatggagg ttctctggat caagtcctga agaaagctgg aagaattcct
      541 gaacaaattt taggaaaagt tagcattgct gtaataaaag gcctgacata tctgagggag
      601 aagcacaaga tcgcggccag agatgtcaag ccctccaaca tcctagtcaa ctcccgtggg
      661 gagatcaagc tctgtgactt tggggtcagc gggcagctca tcgactccat ggccaactcc
      721 ttcgtgggca caaggtccta cgcgtcgcca gaaagactcc aggggactgc ttactctgtg
      781 cagtcagaca tctggagcat gggactgtct ctggtagaga tggcggttgg gaggtatccc
      841 atccctcctc cagatgccaa ggagctggag ctgatgtttg ggtgccaggt ggaaggagat
      901 gcggctgaga ccccacccag gccaaggacc cccgggaggc cccttagctc atacggaatg
      961 gacagccgac ctcccatggc aatttttgag ttgttggatt acatagtcaa cgagcctcct
     1021 ccaaaactgc ccagtggag
//
