LOCUS       sequence-86738-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 576, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AsRed2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1172
                     /codon_start=1
                     /product="Anemonia sulcata red fluorescent protein"
                     /label=AsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASLLKKTMPFRTTIEGTVNGHYFKCTGKGEGNPLEGTQEMKIEV
                     IEGGPLPFAFHILSTSCMYGSKAFIKYVSGIPDYFKQSLPEGFTWERTTTYEDGGFLTA
                     HQDTSLDGDCLVYKVKILGNNFPADGPVMQNKAGRWEPSTEIVYEVDGVLRGQSLMALE
                     CPGGRHLTCHLHTTYRSKKPASALKMPGFHFEDHRIEILEEVEKGKCYKQYEAAVGRYC
                     DAAPSKLGHN"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggcct
      481 ctttgctgaa gaagaccatg cccttcagga ccaccatcga gggcaccgtg aacggccact
      541 acttcaagtg caccggcaag ggcgagggca accccctgga gggcacccag gagatgaaga
      601 tcgaggtgat cgagggcggc cccctgccct tcgccttcca catcctgtcc acctcctgca
      661 tgtacggctc caaggccttc atcaagtacg tgtccggcat ccccgactac ttcaagcagt
      721 ccctccccga gggcttcacc tgggagcgca ccaccaccta cgaggacggc ggcttcctga
      781 ccgcccacca ggacacctcc ctggacggcg actgcctggt gtacaaggtg aagatcctgg
      841 gcaacaactt ccccgccgac ggccccgtga tgcagaacaa ggccggccgc tgggagccct
      901 ccaccgagat cgtgtacgag gtggacggcg tgctgcgcgg ccagtccctg atggccctgg
      961 agtgccccgg cggtcgccac ctgacctgcc acctgcacac cacctaccgc tccaagaagc
     1021 ccgcctccgc cctgaagatg cccggcttcc acttcgagga ccaccgcatc gagatcctgg
     1081 aggaggtgga gaagggcaag tgctacaagc agtacgaggc cgccgtgggc cgctactgcg
     1141 acgccgcccc ctccaagctg ggccacaact gaagcggccg cgactctaga tcataatcag
     1201 ccatacc
//
