LOCUS       sequence-86739-e        2030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2030)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mDsRed-Actin-7
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..797
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      121 ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg cgcatggagg
      181 gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag ccctacgagg
      241 gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca
      301 tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc
      361 ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc atgaacttcg
      421 aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc accttcatct
      481 acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga
      541 ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg ctgaagggcg
      601 agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac ttcaagaccg
      661 tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac tccaagctgg
      721 acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac gccgaggccc
      781 gccactccgg ctcccagtcc ggactcagat ctcgagctat ggatgatgat atcgccgcgc
      841 tcgtcgtcga caacggctcc ggcatgtgca aggccggctt cgcgggcgac gatgcccccc
      901 gggccgtctt cccctccatc gtggggcgcc ccaggcacca gggcgtgatg gtgggcatgg
      961 gtcagaagga ttcctatgtg ggcgacgagg cccagagcaa gagaggcatc ctcaccctga
     1021 agtaccccat cgagcacggc atcgtcacca actgggacga catggagaaa atctggcacc
     1081 acaccttcta caatgagctg cgtgtggctc ccgaggagca ccccgtgctg ctgaccgagg
     1141 cccccctgaa ccccaaggcc aaccgcgaga agatgaccca gatcatgttt gagaccttca
     1201 acaccccagc catgtacgtt gctatccagg ctgtgctatc cctgtacgcc tctggccgta
     1261 ccactggcat cgtgatggac tccggtgacg gggtcaccca cactgtgccc atctacgagg
     1321 ggtatgccct cccccatgcc atcctgcgtc tggacctggc tggccgggac ctgactgact
     1381 acctcatgaa gatcctcacc gagcgcggct acagcttcac caccacggcc gagcgggaaa
     1441 tcgtgcgtga cattaaggag aagctgtgct acgtcgccct ggacttcgag caagagatgg
     1501 ccacggctgc ttccagctcc tccctggaga agagctacga gctgcctgac ggccaggtca
     1561 tcaccattgg caatgagcgg ttccgctgcc ctgaggcact cttccagcct tccttcctgg
     1621 gcatggagtc ctgtggcatc cacgaaacta ccttcaactc catcatgaag tgtgacgtgg
     1681 acatccgcaa agacctgtac gccaacacag tgctgtctgg cggcaccacc atgtaccctg
     1741 gcattgccga caggatgcag aaggagatca ctgccctggc acccagcaca atgaagatca
     1801 agatcattgc tcctcctgag cgcaagtact ccgtgtggat cggcggctcc atcctggcct
     1861 cgctgtccac cttccagcag atgtggatca gcaagcagga gtatgacgag tccggcccct
     1921 ccatcgtcca ccgcaaatgc ttctagggat ccaccggatc tagataactg atcataatca
     1981 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
//
