LOCUS       sequence-8674-ex       11548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Pfeiffer BD, Jenett A, Hammonds AS, Ngo TT, Misra S, Murphy C, 
            Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, 
            Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM.
  TITLE     Tools for Neuroanatomy and Neurogenetics in Drosophila
  JOURNAL   Tools for neuroanatomy and neurogenetics in Drosophila
  PUBMED    18621688
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tools for 
            neuroanatomy and neurogenetics in Drosophila"
COMMENT     Sequence Label: pBPGw
FEATURES             Location/Qualifiers
     source          1..11548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1903..4548
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5044..5060)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5044..5060)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(6071..6685,6756..6887,7091..7406,7468..8122,
                     8197..8470,8872..8943))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9395..9413
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9638..9707
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9794..10382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9874..9893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10553..11344,11345..11413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11176..11195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11414..11518)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccagatctgg taccagatcc gggatcccgt aagcaaataa acaagcgcag ctgaacaagc
     1861 taaacaatct gcagcccaag cttgaagcaa gcctcctgaa agatgaagct actgtcttct
     1921 atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg
     1981 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg
     2041 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag
     2101 ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta
     2161 caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc
     2221 gtcacagata gattggcttc agtggagact gatatgcctc taacattgag acagcataga
     2281 ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta
     2341 tcgattgact cggcagctca tcatgataac tccacaattc cgttggattt tatgcccagg
     2401 gatgctcttc atggatttga ttggtctgaa gaggatgaca tgtcggatgg cttgcccttc
     2461 ctgaaaacgg accccaacaa taatgggttc tttggcgacg gttctctctt atgtattctt
     2521 cgatctattg gctttaaacc ggaaaattac acgaactcta acgttaacag gctcccgacc
     2581 atgattacgg atagatacac gttggcttct agatccacaa catcccgttt acttcaaagt
     2641 tatctcaata attttcaccc ctactgccct atcgtgcact caccgacgct aatgatgttg
     2701 tataataacc agattgaaat cgcgtcgaag gatcaatggc aaatcctttt taactgcata
     2761 ttagccattg gagcctggtg tatagagggg gaatctactg atatagatgt tttttactat
     2821 caaaatgcta aatctcattt gacgagcaag gtcttcgagt caggttccat aattttggtg
     2881 acagccctac atcttctgtc gcgatataca cagtggaggc agaaaacaaa tactagctat
     2941 aattttcaca gcttttccat aagaatggcc atatcattgg gcttgaatag ggacctcccc
     3001 tcgtccttca gtgatagcag cattctggaa caaagacgcc gaatttggtg gtctgtctac
     3061 tcttgggaga tccaattgtc cctgctttat ggtcgatcca tccagctttc tcagaataca
     3121 atctccttcc cttcttctgt cgacgatgtg cagcgtacca caacaggtcc caccatatat
     3181 catggcatca ttgaaacagc aaggctctta caagttttca caaaaatcta tgaactagac
     3241 aaaacagtaa ctgcagaaaa aagtcctata tgtgcaaaaa aatgcttgat gatttgtaat
     3301 gagattgagg aggtttcgag acaggcacca aagtttttac aaatggatat ttccaccacc
     3361 gctctaacca atttgttgaa ggaacaccct tggctatcct ttacaagatt cgaactgaag
     3421 tggaaacagt tgtctcttat catttatgta ttaagagatt ttttcactaa ttttacccag
     3481 aaaaagtcac aactagaaca ggatcaaaat gatcatcaaa gttatgaagt taaacgatgc
     3541 tccatcatgt taagcgatgc agcacaaaga actgttatgt ctgtaagtag ctatatggac
     3601 aatcataatg tcaccccata ttttgcctgg aattgttctt attacttgtt caatgcagtc
     3661 ctagtaccca taaagactct actctcaaac tcaaaatcga atgctgagaa taacgagacc
     3721 gcacaattat tacaacaaat taacactgtt ctgatgctat taaaaaaact ggccactttt
     3781 aaaatccaga cttgtgaaaa atacattcaa gtactggaag aggtatgtgc gccgtttctg
     3841 ttatcacagt gtgcaatccc attaccgcat atcagttata acaatagtaa tggtagcgcc
     3901 attaaaaata ttgtcggttc tgcaactatc gcccaatacc ctactcttcc ggaggaaaat
     3961 gtcaacaata tcagtgttaa atatgtttct cctggctcag tagggccttc acctgtgcca
     4021 ttgaaatcag gagcaagttt cagtgatcta gtcaagctgt tatctaaccg tccaccctct
     4081 cgtaactctc cagtgacaat accaagaagc acaccttcgc atcgctcagt cacgcctttt
     4141 ctagggcaac agcaacagct gcaatcatta gtgccactga ccccgtctgc tttgtttggt
     4201 ggcgccaatt ttaatcaaag tgggaatatt gctgatagct cattgtcctt cactttcact
     4261 aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc gctttcacaa
     4321 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     4381 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     4441 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     4501 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtaaaa tgaatcgtag
     4561 atactgaaaa accccgcaag ttcacttcaa ctgtgcatcg tgcaccatct caatttcttt
     4621 catttataca tcgttttgcc ttcttttatg taactatact cctctaagtt tcaatcttgg
     4681 ccatgtaacc tctgatctat agaatttttt aaatgactag aattaatgcc catctttttt
     4741 ttggacctaa attcttcatg aaaatatatt acgagggctt attcagaagc ttatcgatac
     4801 cgtcgactaa agccaaatag aaaattattc agttcctggc ttaagttttt aaaagtgata
     4861 ttatttattt ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag
     4921 ttttaagtta gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata
     4981 tatttattta aagataattg cgtttttatt gtcagggagt gagtttgctt aaaaactcgt
     5041 ttagatccac tagttctaga gcggccgctg gccacgggtg cgcatgatcg tgctcctgtc
     5101 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     5161 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     5221 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     5281 cattatgttc cggaggcgcg ccctagttcc agtgaaatcc aagcattttc taaattaaat
     5341 gtattcttat tattatagtt gttatttttg atatatataa acaacactat tatgcccacc
     5401 atttttttga gatgcatcta cacaaggaac aaacactgga tgtcactttc agttcaaatt
     5461 gtaacgctaa tcactccgaa caggtcacaa aaaattacct taaaaagtca taatattaaa
     5521 ttagaataaa tatagctgtg agggaaatat atacaaatat attggagcaa ataaattgta
     5581 catacaaata tttattacta atttctattg agacgaaatg aaccactcgg aaccatttga
     5641 gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa
     5701 tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg
     5761 aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg
     5821 tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg
     5881 gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc
     5941 tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc
     6001 aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga
     6061 tatatgtcgg ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc
     6121 ggaagctcac gatgagaatg gccagaccca cgtagtccag cggcagatcg gcggcggaga
     6181 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
     6241 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
     6301 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
     6361 agaagaagcc gccaaagagc aggaatggta tgataaccgg cggacccaca gacagcgcca
     6421 tcgaggtcga ggagctggcg caggatatta gatatccgaa ggacgttgac acattggcca
     6481 ccagagtgac cagcgccagg cagttgaaga agtgcagcac tccggcccgc agtccgatca
     6541 tcggataggc aatcgccgtg aagaccagtg gcactgtgag aaaaagcggc aattcggcaa
     6601 tcgttttgcc cagaaagtat gtgtcacagc gataaagtcg acttcgggcc tccctcataa
     6661 aaactggcag ctctgaggtg aacacctaaa tcgaatcgat tcattagaaa gttagtaaat
     6721 tattgaaatg caaatgtatt ctaaacatga cttacattta tcgtggcaaa gacgttttga
     6781 aaggtcatgt tggtcaggaa gaggaagatg gctccgttga tattcatcac acccacttgc
     6841 gtgagttgtt ggcccaaaaa gatgaggcca atcaagatgg caaccatctg caaattaaaa
     6901 tgttactcgc atctcattaa tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa
     6961 ctataaacta tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctat
     7021 ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg
     7081 aaccactcac cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg
     7141 acagccagga tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt
     7201 acccattctc cggctgctcc agtggcttct ccagattttt ggtggccaac aactgctcca
     7261 tatcccgggc tactttgcta atggcaaaat tgtcgcatat cttggcgatc cgatcacggg
     7321 actcgatctc ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt
     7381 agttggtagg acactgggca cccacgctgg ataggagttg agatgttatg taatactaga
     7441 tacccttaat aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg
     7501 cccaagaaag ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc
     7561 tcggaagacg gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc
     7621 agcacctgga cgacgctgtg ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag
     7681 atcagaagcg gcggatcggt tagagcctcg gaggcgaatg ccagacgctt cctttctccg
     7741 ccggacagac ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc
     7801 gaaagctcct ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt
     7861 ggcatccgca ccatggcttg gaaaatcagg tgttccctgg ccgttaggga gccgataaag
     7921 aggtcatcct gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg
     7981 ccattgagca gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca
     8041 agggcattca gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg
     8101 cccggatagg ccacgccgca aactgagttt caaattggta attggaccct ttattaagat
     8161 ttcacacaga tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg
     8221 gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc
     8281 atccggagcc cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga
     8341 ggttctcggc tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggccgga
     8401 gcgtgccgta gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc
     8461 cgctgtcacc ctgcaatgat aggggatctc aaatatcaac tactagcgtt atgctcatct
     8521 aaccccgaac aaaaagtacc ccgaagtatc ctacgaagta ggtttatact tttatttatt
     8581 ttttgtgcat ctaggatcag cttaaaatat ctggttgtta tattttttgt aaaaaagaat
     8641 atagtcgaaa atgaatgcct ttagatgtct tgatcatgat atgatctcaa aaattgtctt
     8701 atatagcgag aacagctacc agaataatct gtttcgtgtc actatttgtt tgtgcaattg
     8761 cggtttggga tttttgtggg tcgcagttct cacgccgcat acaatttgat gttgcaatcg
     8821 cagttcctat agatcaagtg aacttaagat gtatgcacat gtactactca cattgttcag
     8881 atgctcggca gatgggtgtt tgctgcctcc gcgaattaat agctcctgat cctcttggcc
     8941 cattgccggg atttttcaca ctttcccctg cttacccacc caaaaccaat caccacccca
     9001 atcactcaaa aaacaaacaa aaataagaag cgagaggagt tttggcacag cactttgtgt
     9061 ttaattgatg gcgtaaaccg cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg
     9121 aaggcggaca ttgacgctac gtaacgctac aaacggtggc gaaagagata gcggacgcag
     9181 cggcgaaaga gacggcgata tttctgtgga cagagaagga ggcaaacagc gctgactttg
     9241 agtggaatgt cattttgagt gagaggtaat cgaaagaacc tggtacatca aatacccttg
     9301 gatcgaagta aatttaaaac tgatcagata agttcaatga tatccagtgc agtaaaaaaa
     9361 aaaaatgttt tttttatcta ctttccgcaa aaatgggttt tattaactta catacatact
     9421 aggcgcgccc atatgttcgg cttgtcgaca tgcccgccgt gaccgtcgag aacccgctga
     9481 cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca
     9541 ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca
     9601 actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc
     9661 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
     9721 tcgtcgacgg taacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9841 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9901 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9961 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10021 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10081 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10141 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10201 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10261 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10321 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10381 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10441 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10501 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10561 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10621 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10681 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10741 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10801 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10861 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10921 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10981 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11041 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11101 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11161 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11221 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11281 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11341 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11401 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11461 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11521 catttccccg aaaagtgcca cctgagtc
//
