LOCUS       sequence-86741-e        1381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1381)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mDsRed-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..129
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     80..100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      419..428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             425..1102
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     823..842
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1225..1346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1262..1281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1316..1335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
       61 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      121 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg aggcttcggg agccgctcct
      181 gagcggcagc gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc tgctcgtggc
      241 cgtctgcgct ctgcaccttg gcgtcaccct cgtttactac ctggctggcc gcgacctgag
      301 ccgcctgccc caactggtcg gagtctccac accgctgcag ggcggctcga acagtgccgc
      361 cgccatcggg cagtcctccg gggagctccg gaccggaggg gccaaggatc caccggtcgc
      421 caccatggac aacaccgagg acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga
      481 gggctccgtg aacggccact acttcgagat cgagggcgag ggcgagggca agccctacga
      541 gggcacccag accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga
      601 catcctgtcc ccccagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      661 ccccgactac atgaagctgt ccttccccga gggcttcacc tgggagcgct ccatgaactt
      721 cgaggacggc ggcgtggtgg aggtgcagca ggactcctcc ctgcaggacg gcaccttcat
      781 ctacaaggtg aagttcaagg gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa
      841 gactgccggc tgggagccct ccaccgagaa gctgtacccc caggacggcg tgctgaaggg
      901 cgagatctcc cacgccctga agctgaagga cggcggccac tacacctgcg acttcaagac
      961 cgtgtacaag gccaagaagc ccgtgcagct gcccggcaac cactacgtgg actccaagct
     1021 ggacatcacc aaccacaacg aggactacac cgtggtggag cagtacgagc acgccgaggc
     1081 ccgccactcc ggctcccagt agagcggccg cgactctaga tcataatcag ccataccaca
     1141 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     1201 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     1261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1321 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     1381 a
//
