LOCUS       sequence-86742-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mDsRed-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..454
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             473..1150
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     871..890
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
       61 cgacggtacc gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
      121 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
      181 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
      241 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
      301 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
      361 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
      421 tactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgcca ccatggacaa
      481 caccgaggac gtcatcaagg agttcatgca gttcaaggtg cgcatggagg gctccgtgaa
      541 cggccactac ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac
      601 cgccaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      661 ccagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactacat
      721 gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc atgaacttcg aggacggcgg
      781 cgtggtggag gtgcagcagg actcctccct gcaggacggc accttcatct acaaggtgaa
      841 gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga ctgccggctg
      901 ggagccctcc accgagaagc tgtaccccca ggacggcgtg ctgaagggcg agatctccca
      961 cgccctgaag ctgaaggacg gcggccacta cacctgcgac ttcaagaccg tgtacaaggc
     1021 caagaagccc gtgcagctgc ccggcaacca ctacgtggac tccaagctgg acatcaccaa
     1081 ccacaacgag gactacaccg tggtggagca gtacgagcac gccgaggccc gccactccgg
     1141 ctcccagtaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     1201 tacttgc
//
