LOCUS       sequence-86743-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DsRed2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..921
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(424..444)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     836..859
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctggtacccc ttgcagcttc cagttgtcag gaagagaagg ctcccatgga tcctgtggtg
      121 gtgctggggc tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg
      181 ggagggaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      241 accatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag
      301 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      361 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      421 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
      481 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      541 gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
      601 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat gcagaagaag
      661 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      721 gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
      781 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga cgccaagctg
      841 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
      901 cgccaccacc tgttcctgta agcggccgcg actctagatc ataatcagcc ataccacatt
      961 tgtagaggtt ttacttgctt taaaaaacct ccca
//
