LOCUS       sequence-86748-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DsRed2-Nucleus-4
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..76
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     27..47
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..795
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(301..321)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     713..736
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             811..831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             835..855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             859..879
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
       61 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
      121 atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc
      181 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      301 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      361 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
      481 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc
      541 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      601 acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
      661 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac
      721 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
      781 caccacctgt tcctgagatc tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa
      841 aagaagagaa aggtagatcc aaaaaagaag agaaaggtag gatccaccgg atctagataa
      901 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
      961 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1021 gcagcttata atggttacaa ataaagcaat
//
