LOCUS       sequence-8675-ex       11698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Pfeiffer BD, Jenett A, Hammonds AS, Ngo TT, Misra S, Murphy C, 
            Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, 
            Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM.
  TITLE     Tools for Neuroanatomy and Neurogenetics in Drosophila
  JOURNAL   Tools for neuroanatomy and neurogenetics in Drosophila
  PUBMED    18621688
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tools for 
            neuroanatomy and neurogenetics in Drosophila"
COMMENT     Sequence Label: pBPGUw
FEATURES             Location/Qualifiers
     source          1..11698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2052..4697
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5193..5209)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5193..5209)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(6220..6834,6905..7036,7240..7555,7617..8271,
                     8346..8619,9021..9092))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9544..9562
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9787..9856
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9943..10531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10023..10042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10702..11493,11494..11562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11325..11344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11563..11667)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accagatccg ggatcccgta
     1981 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagcccaagc ttgaagcaag
     2041 cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
     2101 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     2161 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
     2221 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
     2281 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     2341 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     2401 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     2461 gtaacaaagg tcaaagacag ttgactgtat cgattgactc ggcagctcat catgataact
     2521 ccacaattcc gttggatttt atgcccaggg atgctcttca tggatttgat tggtctgaag
     2581 aggatgacat gtcggatggc ttgcccttcc tgaaaacgga ccccaacaat aatgggttct
     2641 ttggcgacgg ttctctctta tgtattcttc gatctattgg ctttaaaccg gaaaattaca
     2701 cgaactctaa cgttaacagg ctcccgacca tgattacgga tagatacacg ttggcttcta
     2761 gatccacaac atcccgttta cttcaaagtt atctcaataa ttttcacccc tactgcccta
     2821 tcgtgcactc accgacgcta atgatgttgt ataataacca gattgaaatc gcgtcgaagg
     2881 atcaatggca aatccttttt aactgcatat tagccattgg agcctggtgt atagaggggg
     2941 aatctactga tatagatgtt ttttactatc aaaatgctaa atctcatttg acgagcaagg
     3001 tcttcgagtc aggttccata attttggtga cagccctaca tcttctgtcg cgatatacac
     3061 agtggaggca gaaaacaaat actagctata attttcacag cttttccata agaatggcca
     3121 tatcattggg cttgaatagg gacctcccct cgtccttcag tgatagcagc attctggaac
     3181 aaagacgccg aatttggtgg tctgtctact cttgggagat ccaattgtcc ctgctttatg
     3241 gtcgatccat ccagctttct cagaatacaa tctccttccc ttcttctgtc gacgatgtgc
     3301 agcgtaccac aacaggtccc accatatatc atggcatcat tgaaacagca aggctcttac
     3361 aagttttcac aaaaatctat gaactagaca aaacagtaac tgcagaaaaa agtcctatat
     3421 gtgcaaaaaa atgcttgatg atttgtaatg agattgagga ggtttcgaga caggcaccaa
     3481 agtttttaca aatggatatt tccaccaccg ctctaaccaa tttgttgaag gaacaccctt
     3541 ggctatcctt tacaagattc gaactgaagt ggaaacagtt gtctcttatc atttatgtat
     3601 taagagattt tttcactaat tttacccaga aaaagtcaca actagaacag gatcaaaatg
     3661 atcatcaaag ttatgaagtt aaacgatgct ccatcatgtt aagcgatgca gcacaaagaa
     3721 ctgttatgtc tgtaagtagc tatatggaca atcataatgt caccccatat tttgcctgga
     3781 attgttctta ttacttgttc aatgcagtcc tagtacccat aaagactcta ctctcaaact
     3841 caaaatcgaa tgctgagaat aacgagaccg cacaattatt acaacaaatt aacactgttc
     3901 tgatgctatt aaaaaaactg gccactttta aaatccagac ttgtgaaaaa tacattcaag
     3961 tactggaaga ggtatgtgcg ccgtttctgt tatcacagtg tgcaatccca ttaccgcata
     4021 tcagttataa caatagtaat ggtagcgcca ttaaaaatat tgtcggttct gcaactatcg
     4081 cccaataccc tactcttccg gaggaaaatg tcaacaatat cagtgttaaa tatgtttctc
     4141 ctggctcagt agggccttca cctgtgccat tgaaatcagg agcaagtttc agtgatctag
     4201 tcaagctgtt atctaaccgt ccaccctctc gtaactctcc agtgacaata ccaagaagca
     4261 caccttcgca tcgctcagtc acgccttttc tagggcaaca gcaacagctg caatcattag
     4321 tgccactgac cccgtctgct ttgtttggtg gcgccaattt taatcaaagt gggaatattg
     4381 ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa
     4441 ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact
     4501 tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt
     4561 cacctggttg gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta
     4621 ataccactac aatggatgat gtatataact atctattcga tgatgaagat accccaccaa
     4681 acccaaaaaa agagtaaaat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
     4741 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     4801 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     4861 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     4921 cgagggctta ttcagaagct tatcgatacc gtcgactaaa gccaaataga aaattattca
     4981 gttcctggct taagttttta aaagtgatat tatttatttg gttgtaacca accaaaagaa
     5041 tgtaaataac taatacataa ttatgttagt tttaagttag caacaaattg attttagcta
     5101 tattagctac ttggttaata aatagaatat atttatttaa agataattgc gtttttattg
     5161 tcagggagtg agtttgctta aaaactcgtt tagatccact agttctagag cggccgctgg
     5221 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
     5281 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
     5341 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
     5401 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggaggcgcgc cctagttcca
     5461 gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg ttatttttga
     5521 tatatataaa caacactatt atgcccacca tttttttgag atgcatctac acaaggaaca
     5581 aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac aggtcacaaa
     5641 aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga gggaaatata
     5701 tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa tttctattga
     5761 gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac taacgacagt
     5821 cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga
     5881 aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga
     5941 aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat
     6001 gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg
     6061 ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa
     6121 atataattac attagctttt tgagggggca ataaacagta aacacgatgg taataatggt
     6181 aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg
     6241 aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagacccac
     6301 gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga ccttgcccga
     6361 actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca cgtccgccca
     6421 ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg acaaccattt
     6481 gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca ggaatggtat
     6541 gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc aggatattag
     6601 atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc agttgaagaa
     6661 gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga agaccagtgg
     6721 cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg tgtcacagcg
     6781 ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga acacctaaat
     6841 cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc taaacatgac
     6901 ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag aggaagatgg
     6961 ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag atgaggccaa
     7021 tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat attcgcgagt
     7081 taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt gaaaaaaact
     7141 aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt tccaagaatt
     7201 gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa taagtcgcac
     7261 ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga ccgcccggaa
     7321 ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca gtggcttctc
     7381 cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa tggcaaaatt
     7441 gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca caacggccaa
     7501 cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac ccacgctgga
     7561 taggagttga gatgttatgt aatactagat acccttaata aacacatcga actcactagg
     7621 aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc tcggccatca
     7681 gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg gtcaggatga
     7741 cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg gcggtaaagg
     7801 agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt agagcctcgg
     7861 aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg ccgggcacac
     7921 cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga tccacgcggg
     7981 ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg aaaatcaggt
     8041 gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag gcgcacctgg
     8101 cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg gatggcgata
     8161 cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc tttccggcac
     8221 cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa actgagtttc
     8281 aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg cgaatagaaa
     8341 ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt tgcagaatag
     8401 tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga ccgccccaaa
     8461 gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc tccctgaacc
     8521 ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct gcccgaagcc
     8581 ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata ggggatctca
     8641 aatatcaact actagcgtta tgctcatcta accccgaaca aaaagtaccc cgaagtatcc
     8701 tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc ttaaaatatc
     8761 tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt tagatgtctt
     8821 gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca gaataatctg
     8881 tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt cgcagttctc
     8941 acgccgcata caatttgatg ttgcaatcgc agttcctata gatcaagtga acttaagatg
     9001 tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt gctgcctccg
     9061 cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac tttcccctgc
     9121 ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa aataagaagc
     9181 gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc ttggagcttc
     9241 gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg taacgctaca
     9301 aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat ttctgtggac
     9361 agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg agaggtaatc
     9421 gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact gatcagataa
     9481 gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac tttccgcaaa
     9541 aatgggtttt attaacttac atacatacta ggcgcgccca tatgttcggc ttgtcgacat
     9601 gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac ccgccgacgc
     9661 cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg gcgagggctt
     9721 cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt tcatcatgat
     9781 ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc cctggcaccc
     9841 gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt aacatgtgag caaaaggcca
     9901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10381 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10681 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10741 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10801 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10861 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10921 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10981 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11041 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11101 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11161 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11221 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11281 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11341 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11401 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11461 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11521 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11581 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11641 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
