LOCUS       sequence-86750-e        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HcRed1-H2B-6
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..1161
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agcggcctgc tgaaggagag tatgcgcatc aagatgtaca tggagggcac cgtgaacggc
      541 cactacttca agtgcgaggg cgagggcgac ggcaacccct tcgccggcac ccagagcatg
      601 agaatccacg tgaccgaggg cgcccccctg cccttcgcct tcgacatcct ggccccctgc
      661 tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg agatccccga cttcttcaag
      721 cagagcttcc ccgagggctt cacctgggag agaaccacca cctacgagga cggcggcatc
      781 ctgaccgccc accaggacac cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg
      841 cacggcacca acttccccgc cgacggcccc gtgatgaaga acaagagcgg cggctgggag
      901 cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc
      961 ctgaaggtgg gcgaccggca cctgatctgc caccactaca ccagctaccg gagcaagaag
     1021 gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg acatccggct ccagatgctg
     1081 cggaagaaga aggacgagta cttcgagctg tacgaggcca gcgtggcccg gtacagcgac
     1141 ctgcccgaga aggccaacta agcggccgcg actctagatc ataatcagcc ataccacatt
//
