LOCUS       sequence-86751-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HcRed1-Mito-7
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..73
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     24..44
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             102..176
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      204..213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             210..896
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
       61 tataagcaga gctggtttag tgaaccgtca gatccgctag catgtccgtc ctgacgccgc
      121 tgctgctgcg gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc
      181 attcgttggg ggatccaccg gtcgccacca tggtgagcgg cctgctgaag gagagtatgc
      241 gcatcaagat gtacatggag ggcaccgtga acggccacta cttcaagtgc gagggcgagg
      301 gcgacggcaa ccccttcgcc ggcacccaga gcatgagaat ccacgtgacc gagggcgccc
      361 ccctgccctt cgccttcgac atcctggccc cctgctgcga gtacggcagc aggaccttcg
      421 tgcaccacac cgccgagatc cccgacttct tcaagcagag cttccccgag ggcttcacct
      481 gggagagaac caccacctac gaggacggcg gcatcctgac cgcccaccag gacaccagcc
      541 tggagggcaa ctgcctgatc tacaaggtga aggtgcacgg caccaacttc cccgccgacg
      601 gccccgtgat gaagaacaag agcggcggct gggagcccag caccgaggtg gtgtaccccg
      661 agaacggcgt gctgtgcggc cggaacgtga tggccctgaa ggtgggcgac cggcacctga
      721 tctgccacca ctacaccagc taccggagca agaaggccgt gcgcgccctg accatgcccg
      781 gcttccactt caccgacatc cggctccaga tgctgcggaa gaagaaggac gagtacttcg
      841 agctgtacga ggccagcgtg gcccggtaca gcgacctgcc cgagaaggcc aactgaagcg
      901 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      961 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1021 cttgtttatt gcagcttata atggt
//
