LOCUS       sequence-86752-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HcRed1-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..806
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             831..851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             855..875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             879..899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      121 ccatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg gagggcaccg
      181 tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc gccggcaccc
      241 agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc gacatcctgg
      301 ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag atccccgact
      361 tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc tacgaggacg
      421 gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg atctacaagg
      481 tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac aagagcggcg
      541 gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc ggccggaacg
      601 tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc agctaccgga
      661 gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac atccggctcc
      721 agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc gtggcccggt
      781 acagcgacct gcccgagaag gccaactccg gactcagatc tcgagctgat ccaaaaaaga
      841 agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag
      901 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      961 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1021 ttgttgttaa cttgtttatt gcagcttata
//
