LOCUS       sequence-86755-e        3135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3135)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..714
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     regulatory      769..778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             820..855
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgaaca gcctgatcaa agaaaacatg
       61 cggatgaagg tggtgctgga aggcagcgtg aacggccacc agttcaagtg caccggcgag
      121 ggcgagggca acccctacat gggcacccag accatgcgga tcaaagtgat cgagggcgga
      181 cctctgccct tcgccttcga catcctggcc acatccttca tgtacggcag ccggaccttc
      241 atcaagtacc ccaagggcat ccccgatttc ttcaagcaga gcttccccga gggcttcacc
      301 tgggagagag tgaccagata cgaggacggc ggcgtgatca ccgtgatgca ggacaccagc
      361 ctggaagatg gctgcctggt gtaccatgcc caggtcaggg gcgtgaattt tcccagcaac
      421 ggcgccgtga tgcagaagaa aaccaagggc tgggagccca acaccgagat gatgtacccc
      481 gctgacggcg gactgagagg ctacacccac atggccctga aggtggacgg cggagggcac
      541 ctgagctgca gcttcgtgac cacctaccga tccaagaaaa ccgtgggcaa catcaagatg
      601 cccggcatcc acgccgtgga ccaccggctg gaaaggctgg aagagtccga caacgagatg
      661 ttcgtggtgc agcgggagca cgccgtggcc aagttcgccg gcctgggcgg agggtccgga
      721 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggc caccatggct
      781 actcaagctg acctgatgga gttggacatg gccatggagc cggacagaaa agctgctgtc
      841 agccactggc agcagcagtc ttacttggat tctggaatcc attctggtgc caccaccaca
      901 gctccttccc tgagtggcaa gggcaaccct gaggaagaag atgttgacac ctcccaagtc
      961 ctttatgaat gggagcaagg cttttcccag tccttcacgc aagagcaagt agctgatatt
     1021 gacgggcagt atgcaatgac tagggctcag agggtccgag ctgccatgtt ccctgagacg
     1081 ctagatgagg gcatgcagat cccatccacg cagtttgacg ctgctcatcc cactaatgtc
     1141 cagcgcttgg ctgaaccatc acagatgttg aaacatgcag ttgtcaattt gattaactat
     1201 caggatgacg cggaacttgc cacacgtgca attcctgagc tgacaaaact gctaaacgat
     1261 gaggaccagg tggtagttaa taaagctgct gttatggtcc atcagctttc caaaaaggaa
     1321 gcttccagac atgccatcat gcgctcccct cagatggtgt ctgccattgt acgcaccatg
     1381 cagaatacaa atgatgtaga gacagctcgt tgtactgctg ggactctgca caacctttct
     1441 caccaccgcg agggcttgct ggccatcttt aagtctggtg gcatcccagc gctggtgaaa
     1501 atgcttgggt caccagtgga ttctgtactg ttctacgcca tcacgacact gcataatctc
     1561 ctgctccatc aggaaggagc taaaatggca gtgcgcctag ctggtggact gcagaaaatg
     1621 gttgctttgc tcaacaaaac aaacgtgaaa ttcttggcta ttacaacaga ctgccttcag
     1681 atcttagctt atggcaatca agagagcaag ctcatcattc tggccagtgg tggaccccaa
     1741 gccttagtaa acataatgag gacctacact tatgagaagc ttctgtggac cacaagcaga
     1801 gtgctgaaag tgctgtctgt ctgctctagc aacaagccgg ccattgtaga agctggtggg
     1861 atgcaggcac tggggcttca tctgacagac ccaagtcagc gacttgttca aaactgtctt
     1921 tggactctca gaaacctttc agatgcagcg actaagcagg aagggatgga aggcctcctt
     1981 gggactctag tgcagcttct gggttccgat gatataaatg tggtcacctg tgcagctgga
     2041 attctctcta acctcacttg caataattac aaaaacaaga tgatggtgtg ccaagtgggt
     2101 ggcatagagg ctcttgtacg caccgtcctt cgtgctggtg acagggaaga catcactgag
     2161 cctgccatct gtgctcttcg tcatctgacc agccggcatc aggaagccga gatggcccag
     2221 aatgccgttc gccttcatta tggactgcct gttgtggtta aactcctgca cccaccatcc
     2281 cactggcctc tgataaaggc aactgttgga ttgattcgaa accttgccct ttgcccagca
     2341 aatcatgcgc ctttgcggga acagggtgct attccacgac tagttcagct gcttgtacga
     2401 gcacatcagg acacccaacg gcgcacctcc atgggtggaa cgcagcagca gtttgtggag
     2461 ggcgtgcgca tggaggagat agtagaaggg tgtactggag ctctccacat ccttgctcgg
     2521 gacgttcaca accggattgt aatccgagga ctcaatacca ttccattgtt tgtgcagttg
     2581 ctttattctc ccattgaaaa tatccaaaga gtagctgcag gggtcctctg tgaacttgct
     2641 caggacaagg aggctgcaga ggccattgaa gctgagggag ccacagctcc cctgacagag
     2701 ttactccact ccaggaatga aggcgtggca acatacgcag ctgctgtcct attccgaatg
     2761 tctgaggaca agccacagga ttacaagaag cggctttcag tcgagctgac cagttccctc
     2821 ttcaggacag agccaatggc ttggaatgag actgcagatc ttggactgga cattggtgcc
     2881 cagggagaag cccttggata tcgccaggat gatcccagct accgttcttt tcactctggt
     2941 ggatacggcc aggatgcctt ggggatggac cctatgatgg agcatgagat gggtggccac
     3001 caccctggtg ctgactatcc agttgatggg ctgcctgatc tgggacacgc ccaggacctc
     3061 atggatgggc tgcccccagg tgatagcaat cagctggcct ggtttgatac tgacctgtaa
     3121 aagctttgga ttccc
//
